Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1960; 4(03): 299-302DOI: 10.1055/s-0038-1654511 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Ultracentrifugation of Acetylated Thrombin[*] Authors Author Affiliations Walter H. Seegers 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Robert S. Shepard 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Ricardo H. Landaburu 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Recommend Article Abstract Buy Article(opens in new window) Summary Acetylated bovine thrombin with a specific activity of 5930 units per milligram dry weight was found to be homogeneous by ultracentrifuge analysis. The sedimentation constant in units of 10−13 sec. at 20° C, in 0.1 M potassium chloride was 3.3. This constant is not concentration dependent. Full Text References References (1) Landaburu R. H, Seegers W. H. Further studies of prothrombin derivatives. Proc. Soc. exp. Biol. Med. (N. Y.) 94: 708 1957; (2) Landaburu R. H, Seegers W. H. The generation of proteolytic activity during the activation of prothrombin. Amer. J. Physiol. 197: 1178 1959; (3) Seegers W. H, Andrews E. B, McClaughry R. I. Formation of prothrombin derivatives from purified prothrombin. Amer. J. Physiol. 164: 722 1951; (4) Seegers W. H, Thomas W. R, Landaburu R. H, Mammen E. F. Prothrombin: Living enzyme of my blood. Rec. chem. Progress.. In press. (5) Seegers W. H, Landaburu R. H, Johnson J. F. Thrombin-E as a fibrinolytic enzyme. Science 131: 126 1960; (6) Seegers W. H, Levine W. G, Shepard R. S. Further studies on the purification of thrombin. Canad. J. Biochem. 36: 603 1958; (7) Landaburu R. H, Seegers W. H. The activation of thrombin. Canad. J. Biochem. 37: 1362 1959; (8) Seegers W. H, Casillas G, Shepard R. S, Thomas W. R, Halick P. Some properties of thrombin preparations. Canad. J. Biochem. 37: 775 1959;
